SIMULATED MONOLITH™

STYROSä HIC-Phenyl, STYROSä HIC-Butyl, STYROSä HIC-Ether, SIMULATED MONOLITH™ columns for High Speed, High Performance Liquid Chromatography.
High Chromatographic Performance, Chemical Stability, Versatility as well as Cost, were considered in the development of this new line of High Performance Liquid Chromatography Columns.

STYROS™ HIC-Phenyl

Standard Protein Separation on STYROSä HIC Phenyl/XH.

STYROS™ HIC-Butyl

Standard Protein Separation on STYROSä HIC-Butyl/XH.

STYROS™ HIC-Ether

Standard Protein Separation on STYROSä HIC-Ether/XH.

Contents:

• Product Summary 
• Key Features
• Specifications

• Product summary: 

STYROSä HIC series are a new line of Simulated Monolithä packed columns made of polymeric media intended for the chromatography of biomolecules such as proteins and peptides.

The base monolithic matrix that is fully pervious is made of highly crosslinked poly (styrene-divinylbenzene).

The unique macroporous structure of the bed provides a maximum surface contact as well as a uniform flow path, making it possible to run high speed, high-resolution separations without any mass transfer restrictions.

Unlike soft gel, Simulated Monolithä columns can be used at high backpressures, reducing considerably the run time.

Typical linear flow rates are 1,800 cm/hr or above, compared to 140 cm/hr with soft gel.

Unlike Monolith, there are no limitations with Simulated Monolithä columns. They are available in most sizes and configurations.

Higher practical and efficient use of the column is another advantage of such products.

HIC is the method of choice for the purification and separation of biomolecules that have been precipitated with ammonium sulfate or eluted in high salt during ion exchange separations.

Compared to reverse phase, HIC provides a milder technique in using the surface hydrophobicity of biomolecules for their separations. Since no organic solvent is involved, the molecules retain their biological activity.

• Key Features:

Feature 1:

Optimum protein capacity of up to 35 mg/ml 

Feature 2:

Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the beads, we recommend the following pressure limits:

4,000 psi for the XH series,

3,000 psi for the XP and XM series.

4,000 psi for the NB series 

4,000 psi for the MB series

Feature 3:

No measurable shrinking and/or swelling due to highly crosslinked nature of the beads.

• Specifications:

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