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FYI NOTE

FYI042298

STYROS™ Polymeric Stationary Phases as Compared with Silica.

 

Rather than spending hours finding the optimum conditions to separate your proteins, using reversed phase silica:

You could spend minutes using  STYROS™  reversed phase polymerics:

 

And then reduce the flow rate to find out more about additional details, such as the effect of reversed phase media, time or organic solvent, on your proteins:

 

 

Operating parameters: 

HPLC System.

HP 1100, standard cell.

Columns

As indicated on each chromatogram.

Mobile Phase

A: 0.1% TFA in H2O
B: 0.1% TFA in Acetonitrile/H2O (95/5)(v/v)

Gradient

15 to 100 % B in 30 column volumes

Temperature

20°C.

Detection

214 nm

Sample
(1mg/ml each)

Ribonuclease A, Cytochrome C, Lysozyme, BSA, Myoglobin, OVA.

 

Polymeric Stationary Phases, Setting new standards in High Speed, High Performance, Liquid Chromatography.

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