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FYI NOTE

FYI030398

A simple alternative to reduce sample consumption and waste generation by over 40%.


Chromatogram 1
Chromatogram 2

 

Although similar chromatographic results can be obtained by using larger bore columns, Narrow Bore columns with STYROS™ stationary phases can be used to reduce the amount of sample injected as well as the waste generated during separations.

Since the stationary phase has very unique flow characteristics, namely the maintenance of resolution at high flow rates and the tolerance of back pressures as high as 5,000 psi, no additional time is required when using the STYROS™ Narrow Bore series.

Moreover, as shown in the chromatograms, the same instrument set up with a standard flow cell is adequate for such chromatographic runs.

The STYROS™ Narrow Bore series is OraChrom’s answer to today’s business concerns: environmental issues and laboratory costs.

 

Operating parameters:

HPLC System.

HP 1100, standard cell.

Column

As indicated on each chromatogram.

Mobile Phase

A: 0.1% TFA in H2O
B: 0.1% TFA in Acetonitrile/H2O (95/5)(v/v)

Flow rate

Chromatogram 1:  2 ml/min(3,470 cm/h)
Chromatogram 2:  3ml/min (1,080 cm/hr)

Gradient

15 to 100% B in 30 column volumes

Temperature

20°C.

Detection

214 nm

Injection volume

    6ml for 2.1 x 150 mm column
  10 ml for 4.6 x 50 mm column

Sample
(1 mg/ml each)

Ribonuclease, Cytochrome C, Lysozyme, BSA, Hemoglobin, OVA.

Polymeric Stationary Phases, Setting New Standards in High Speed, High Performance, Liquid Chromatography.

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