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APPLICATION NOTE

 

AN080297-02

Fast Protein Separation on STYROSä 1R and 2R.

STYROSä 1 R and 2 R/XH series were designed for fast, high-performance separations of biomolecules.

Both media can typically be run at linear flow rates of 1800 cm/hr and separate mixtures of several proteins.

Chromatograms 1 and 2 provide the separation of 4 proteins: Cytochrome C, Lysozyme, b-Lactoglobulin and Ovalbumin in less than one minute, at room temperature.

Each of the two generations has a specific retentivity that differentiates it from the other. In addition to high selectivity, each stationary phase offers a characteristic protein capacity that provides the end user with more selection.

The design of an optimized pore structure allows full access for large molecules such as proteins, to the inner matrix, making it possible to run the separations at high flow rates without loss of capacity or resolution.

Table 1. HPLC Operating Parameters for Chromatograms 1 and 2.

HPLC System

Hewlett Packard 1050

Detector

214 nm

Column

STYROSÔ 1 R/XH 50x4.6mm ( Chromatogram 1 )
 STYROSÔ 2 R/XH 50x4.6 mm ( Chromatogram 2 )

Mobile Phase

A: 0.1 % TFA in water
B: 0.1 % TFA in Acetonitrile/water 95/5

Gradient

15-80% B in 1 minutes

Flow rate

5 ml/min (1,807 cm/hr).

Temperature

Ambient

Injection volume

10 ml

Sample
1 mg/ml each

1: Cytochrome C, 2: Lysozyme
3: b-Lactoglobulin, 4: Ovalbumin

 

Chromatogram 1:
Standard Protein Separation on STYROSÔ 1 R/XH

 

Chromatogram 2:
Standard Protein Separation on STYROS Ô 2 R/XH


 

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