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SIMULATED MONOLITH™ POLYMERIC STATIONARY PHASES FOR LIQUID CHROMATOGRAPHY | |
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STYROS™
HQ and STYROS™ HPA
are the High Capacity versions of the previously developed
anion exchangers for High Throughput purification of proteins and
peptides.
The base matrix that is fully pervious is made of highly crosslinked
poly(styrene-divinylbenzen).
Our proprietary technique ensures that the beads are not brittle and
as a result, free of any leachables.
The unique macroporous structure takes full advantage of the inner
bead's surface area making it possible to run high speed, high
resolution separation without any mass transfer restriction.
STYROS™ HQ and HPA
provide high static and dynamic capacities for analytical and semi
preparative separations.
They have extremely high pressure tolerance, and can be used up to
5,000 psi.
High resolution separation can be achieved at high, as well as low
flow rates.
The goal has been to provide seamless series of stationary phases
that could facilitate the transition from analytical to semi
preparative and preparative applications, all within the same
products.
Our proprietary column packing are geared towards providing high
batch to batch reproducibility.
High protein capacity (measured with BSA)
90 mg/ml for STYROS™ HQ and
80 mg/ml for STYROS™ PA
Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the beads, we recommend the following pressure limits:
4,000 psi for the XH series,
3,000 psi for the XP and XM series.
4,000 psi for the NB series
4,000 psi for the MB series
No measurable shrinking and/or swelling due to highly crosslinked nature of the beads.
| Support Matrix | Crosslinked Poly(styrene-divinylbenzene) |
| Surface Functionalities STYROS™ HQ STYROS™ HPA |
Quaternary Aminomethyl Polyamine |
| Shipping Solvent | 30 % MeOH in DI Water |
| Packing Density | 0.4 g/ml |
| Shrinkage/Swelling | < 0.5 % under different salt or solvent concentrations |
| Maximum Pressure Tolerance | 270 bar (4,000 psi, 27 MPa) |
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STYROS™
HQ/NB |
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To compare with Monodispersed soft gel 5 x 50 mm. Separation of egg white proteins at 300 cm/hr |