LOGO SIMULATED MONOLITH™ POLYMERIC STATIONARY PHASES FOR LIQUID CHROMATOGRAPHY
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STYROS™ HIC-Phenyl, -Butyl, -Ether, and -t-Butyl

Contents:

STYROSä HIC-Phenyl, STYROSä HIC-Butyl, STYROSä HIC-Ether,STYROSä HIC-t-Butyl SIMULATED MONOLITH™ columns for High Speed, High Performance Liquid Chromatography.
High Chromatographic Performance, Chemical Stability, Versatility as well as Cost, were considered in the development of this new line of High Performance Liquid Chromatography Columns.

The base monolithic matrix that is fully pervious is made of highly crosslinked poly (styrene-divinylbenzene).

melends itself to the separation of biomolecules such as proteins and peptides.

The unique macroporous structure of the bed provides a maximum surface contact as well as a uniform flow path, making it possible to run high speed, high-resolution separations without any mass transfer restrictions.

Unlike soft gel, Simulated Monolithä columns can be used at high backpressures, reducing considerably the run time.

Typical linear flow rates are 1,800 cm/hr or above, compared to 140 cm/hr with soft gel.

Unlike Monolith, there are no limitations with Simulated Monolithä columns.
They are available in most sizes and configurations.

Higher practical and efficient use of the column is another advantage of such products.

HIC is the method of choice for the purification and separation of biomolecules that have been precipitated with ammonium sulfate or eluted in high salt during ion exchange separations.

Compared to reverse phase, HIC provides a milder technique in using the surface hydrophobicity of biomolecules for their separations.

Since no organic solvent is involved, the molecules retain their biological activity.

  • Key Features:

Feature 1:

Optimum protein capacity of up to 35 mg/ml 

Feature 2:

Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the bed, we recommend the following pressure limits:

4,000 psi for the XH series,

3,000 psi for the XP and XM series.

4,000 psi for the NB series 

4,000 psi for the MB series

Feature 3:

No measurable shrinking and/or swelling due to highly crosslinked nature of the matrix.

  • Specifications:

  Support Matrix Crosslinked Poly(styrene-divinylbenzene)  
  Surface Functionalities    
  STYROS™ HIC-Phenyl -CH2-C6H5  
STYROS™ HIC-Butyl -C4H9
  STYROS™ HIC-Ether -CH2-CH2-O-CH3  
  STYROS™ HIC-t-Butyl -C(CH3)3  
  Binding Capacity 35 mg/ml protein  
  Shipping Solvent 30 % MeOH in DI Water  
  Packing Density 0.4 g/ml  
  Shrinkage/Swelling < 0.2 % under different salt or solvent concentrations  
  Maximum Pressure Tolerance 270 bar (4,000 psi,  27 MPa)  

 

Separation of 6 Proteins on
STYROSä Phenyl/XH 4.6 x 250 mm, run at 2ml/min (720 cm/hr)

Comparative Protein Separation on STYROSä HIC-Ether, HIC-Butyl, HIC-Phenyl/XH
on 4.6 x 100 mm columns run at 1 ml/min (360 cm/hr of linear velocity).

Separation of 6 proteins on
STYROSä t-Butyl/XH.on 4.6 x 100 mm columns run at 1 ml/min (360 cm/hr of linear velocity).