![]() |
SIMULATED MONOLITH™ POLYMERIC STATIONARY PHASES FOR LIQUID CHROMATOGRAPHY | |
![]() |
![]() |
|
STYROS™ DEAE
is a monolithic, fully uniform polymer based packing for anion
exchange chromatography of biomolecules such as proteins and
peptides, as well as smaller molecules, intended for use in
high speed, high resolution applications. The base matrix that is
fully pervious is made of highly crosslinked
poly(styrene-divinylbenzene).
Our proprietary polymerization technique ensures that the beads are
not brittle and as a result, free of any leachables.
The unique macroporous structure takes full advantage of the inner
bead's surface area making it possible to run high speed, high
resolution separation without any mass transfer restrictions.
STYROS™ DEAE
provides high static and dynamic capacity for analytical and semi
preparative separations.
It has an extremely high pressure tolerance, and can be used up to
5,000 psi.
High resolution separation can be achieved at high, as well as low
flow rates.
The goal has been to provide seamless series of stationary phases
that could facilitate the transition from analytical to semi
preparative and preparative applications, all within the same
products.
Our proprietary column packing are geared towards providing high
batch to batch reproducibility.
Optimum protein capacity (measured with BSA)
85 mg/ml for STYROS™ DEAE
Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the beads, we recommend the following pressure limits:
4,000 psi for the XH series,
3,000 psi for the XP and XM series.
4,000 psi for the NB series
4,000 psi for the MB series
No measurable shrinking and/or swelling due to highly crosslinked nature of the beads.
| Support Matrix | Crosslinked Poly(styrene-divinylbenzene) |
| Surface
Functionalities STYROS™ DEAE |
Diethyaminoethyl |
| Shipping Solvent | 30 % MeOH in DI Water |
| Packing Density | 0.4 g/ml |
| Shrinkage/Swelling | < 0.5 % under different salt or solvent concentrations |
| Maximum Pressure Tolerance | 270 bar (4,000 psi, 27 MPa) |
![]() |
|
STYROS™ DEAE/NB Separation of Hemoglobin variants at 1,800 cm/hr |
![]() |
|
STYROS™ DEAE/NB Separation of 5 Nucleotides at 1,800 cm/hr (1 ml/min). |