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SIMULATED MONOLITH™ POLYMERIC STATIONARY PHASES FOR LIQUID CHROMATOGRAPHY | |
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STYROS™
CM is a Simulated Monolith fully uniform polymer based
packing for cation exchange chromatography of biomolecules such as
proteins and peptides, intended for use in high speed, high
resolution applications.
The base matrix that is fully pervious is made of highly crosslinked
poly(styrene-divinylbenzene).
Our proprietary polymerization technique ensures that the packing is
not brittle and as a result, free of any leachables.
The unique macroporous structure takes full advantage of the inner
surface area making it possible to run high speed, high
resolution separation without any mass transfer restrictions.
STYROS™ CM provides
high static and dynamic capacities to enable high throughput
separations during
analytical and preparative runs.
It has an very high pressure tolerance, and can be used up to
3,000 psi.
High resolution separations can be achieved at high, as well as low
flow rates.
The goal has been to provide seamless series of stationary phases
that could facilitate the transition from analytical to semi
preparative and preparative applications, all within the same products.
Our proprietary manufactured columns are geared towards providing high
reproducibility.
Feature 1:
Optimum protein capacity (measured with RNase)
55 mg/ml
Feature 2:
Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the beads, we recommend the following pressure limits:
4,000 psi for the XH series,
3,000 psi for the XP and XM series.
4,000 psi for the NB series
Feature 3:
No measurable shrinking and/or swelling due to highly crosslinked nature of the beads.
| Support Matrix | Crosslinked Poly(styrene-divinylbenzene) |
| Surface Functionalities STYROS™ CM |
Carboxymethyl |
| Shipping Solvent | 30 % MeOH in DI Water |
| Packing Density | 0.4 g/ml |
| Shrinkage/Swelling | < 0.5 % under different salt or solvent concentrations |
| Maximum Pressure Tolerance | 270 bar (4,000 psi, 27 MPa) |
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| STYROS™
CM/XH 4.6 x 100 mm Column. Standard separation of 4 proteins at 1,800 cm/hr (5 ml/min) |
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| Same column at 360 cm/hr (1 ml/min) |