STYROS™ Amino HILIC

Contents:

• Product Summary 
• Key Features
• Specifications
• STYROS™ Amino HILIC Price List
  
  

• Product summary: 

STYROS™ Amino HILIC is the first Simulated Monolith™ HILIC column made of hard gel fully pervious polymeric media that has been covalently coated with a monolayer hydrophilic surface.

The unique macroporous structure of the bed provides maximum surface contact as well as uniform flow path, making it possible to run high speed, high-resolution separations without any mass transfer restrictions.

Unlike soft gel, Simulated Monolith™ columns can be used at high backpressures, reducing considerably the run time.

Typical linear flow rates are 300 to 1,200 cm/hr and above, compared to 140 cm/hr with soft gel.

Unlike Monolith, there are no limitations with Simulated Monolith™ columns. They are available in most sizes and configurations.

Higher practical speed and efficient use of the column is another advantage of such products.

About HILIC:

HILIC or Hydrophilic Interaction Chromatography is a variation of normal phase chromatography with the advantage of being able to use solvents that are not miscible with water.

HILIC is also called “reverse reversed phase” or “aqueous normal phase” chromatography.

STYROS™ Amino HILIC provides the possibility of anion exchange chromatography when the polar analytes partition in and out of the aqueous layer.

The mobile phase is made of organic for the most part (>80%) and smaller amounts of aqueous or other polar solvents that constitute the strong eluting solvents.

The stationary phase must be polar.

This mode of chromatography shortens the sample preparation procedures.

In the final step of the isolation of proteins whether using SPE, precipitation or liquid/liquid extraction, the isolated product is in an organic solvent such as acetonitrile, isopropanol or alike.

The organic solvent must then be evaporated and the sample reconstituted in the starting mobile phase prior to using it in reversed phase mode.

The possibility of using the sample in the initial organic solvent on HILIC mode instead, eliminates the evaporation-reconstitution steps and allows the direct use of the organic sample on HILIC.

• Key Features:

Feature 1:

Optimum protein capacity

Feature 2:

Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the bed, we recommend the following pressure limits:

3,000 psi for the XH series, 

3,000 psi for the NB series 

3,000 psi for the MB series

Feature 3:

No measurable shrinking and/or swelling due to highly crosslinked nature of the matrix.

• Specifications:

Support Matrix	Crosslinked Poly(styrene-divinylbenzene)
Surface Functionalities
STYROS™ Amino HILIC	Charged Hydrophilic
Binding Capacity	N/A
Shipping Solvent	30 % MeOH in DI Water
Packing Density	0.4 g/ml
Shrinkage/Swelling	< 0.2 % under different salt or solvent concentrations
Maximum Pressure Tolerance	207 bar (3,000 psi,  21 MPa)

Separation at 1 ml/min (360 cm/hr) on a
4.6 x 100 mm STYROS™ Amino HILIC column.

Separation of 4 Nucleotides at 4 ml/min (1,450 cm/hr) on a 4.6 x 100 mm STYROS™ Amino HILIC column

Separation of Benzoic acid and its derivatives on  STYROS™ Amino HILIC

Loadability study on  STYROS™ Amino HILIC

See Application note 52 for further detail