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SIMULATED MONOLITH™ POLYMERIC STATIONARY PHASES FOR LIQUID CHROMATOGRAPHY | |
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STYROS™
3R
Reversed Phase Simulated Monolith™
Columns for High Speed, High Performance Liquid Chromatography
Contents:
- Product Summary
- Key Features
- Specifications
- STYROS™ 3R Price List
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Product summary:
STYROS™ 3R Reversed Phase is the first Simulated Monolith™ polymeric column made of hard gel fully pervious polymeric media.
The unique macroporous structure of the bed provides maximum surface contact as well as uniform flow path, making it possible to run high speed, high-resolution separations without any mass transfer restrictions.
Unlike soft gel, Simulated Monolith™ columns can be used at high backpressures, reducing considerably the run time.
Typical linear flow rates are 700 to 1,800 cm/hr and above, compared to 140 cm/hr with soft gel.
Unlike Monolith, there are no limitations with Simulated Monolith™ columns. They are available in most sizes and configurations.
Higher practical speed and efficient use of the column is another advantage of such products.
About Hard Gel Fully Pervious Polymeric
Reversed phase chromatography with silica provides high resolving power but very limited stability due to the solubility of the matrix.
The separations are therefore limited to neutral pH’s for a limited number of runs.
Polymeric on the other hand have been used successfully to alleviate the issues of chemical instability associated with silica matrices and therefore have provided the possibility of using the full pH range for baseline separations.
Baseline separations can be achieved at 1,200 cm/hr on a narrow bore column of 2.1 mm ID at 60° C temperatures:
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Key Features:
Feature 1:
High protein capacity (measured with Lysozyme)
10 mg/ml
Feature 2:
Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the bed, we recommend the following pressure limits:
4,000 psi for the XH series,
3,000 psi for the XP and XM series.
4,000 psi for the NB series
4,000 psi for the MB series
Feature 3:
No measurable shrinking and/or swelling due
to highly crosslinked nature of the stationary phase.
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Specifications:
| Support Matrix | Crosslinked Poly(styrene-divinylbenzene) |
| Surface Functionalities | Phenyl/Aryl |
| Shipping Solvent | Acetonitrile/Water 95/5 (v/v) |
| Packing Density | 0.35 g/ml |
| Shrinkage/Swelling | < 0.5 % from 0 to 100 water to organic |
| Maximum Pressure Tolerance | 270 bar (4,000 psi, 27 MPa) |
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| Separation of 1. Thiourea, 2.
Benzyl alcohol, 3. Aniline, 4. Nitrobenzene, 5. Methyl Salicylate, 6. Toluene on a 2.1 x 100 mm STYROS™ 3R Simulated Monolith™ column. (Flow Rate: 0.7 ml/min or 1,200 cm/hr) |
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| Separation of
6
Proteins
on
a 4.6 x 50 mm
STYROS™ 3R Simulated Monolith™
column.
(Flow Rate: 4 ml/min or 1,450 cm/hr) |
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| Separation of
7 Peptides
(1. Gly-Tyr, 2.
Val-Tyr-Val, 3. Met enkephalin, 4. Leu enkephalin, 5.
Angiotensin II, 6. Angiotensin III, 7. Angiotensin I) on a 4.6 x 250 mm STYROS™ 3R Simulated Monolith™ column. (Flow Rate: 1 ml/min or 360 cm/hr) |
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Separation of
Benzoic acid and its
derivatives on STYROS™
3R Simulated Monolith™
column.
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