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SIMULATED MONOLITH™ POLYMERIC STATIONARY PHASES FOR LIQUID CHROMATOGRAPHY | |
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Product summary:
STYROS™ 1R and STYROS™
2R are the first two generations of our reversed-phase
polymeric stationary phases. They offer high protein capacity, high
pressure tolerance and the possibility to be used in scale up
processes since they do not leach and therefore contaminate the
final product.
The fully pervious nature of the media with a unimodal pore
structure enables exceptional mass transport kinetics.
STYROS™ 1R has a
binding strength equivalent to that of a silica C4, whereas the
binding strength of STYROS™
2R is closer to other poly(styrene-divinylbenzene) media such
as POROS® R from PerSeptive Biosystems or PLRP-S from Polymer
Laboratories.
Key Features:
Feature 1:
High protein capacity (measured with Lysozyme)
15 mg/ml for STYROS™ 1R and
10 mg/ml for STYROS™ 2R
Feature 2:
Although we have used pressures of up to 10,000 psi without irreversibly altering the structure of the bed, we recommend the following pressure limits:
4,000 psi for the XH series,
3,000 psi for the XP and XM series.
4,000 psi for the NB series
4,000 psi for the MB series
Feature 3:
No measurable shrinking and/or
swelling due to highly crosslinked nature of the stationary phase.
Specifications:
|
Support Matrix |
Crosslinked Poly(styrene-divinylbenzene) |
|
Surface Functionalities |
Phenyl/Aryl |
|
Shipping Solvent |
Acetonitrile/Water 95/5 (v/v) |
|
Packing Density |
0.35 g/ml |
|
Shrinkage/Swelling |
< 0.5 % from 0 to 100 water to organic |
|
Maximum Pressure Tolerance |
270 bar (4,000 psi, 27 MPa) |
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| Separation of Cytochrome C (1), Lysozyme (2),
ß-Lactoglobulin (3) and Ovalbumin (4) on 4.6 x 50 mm columns STYROS™ 1R (Top chromatogram) and STYROS™ 2R (Bottom chromatogram). |
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| STYROS™
2R/NB (2.1 x 250 mm) 2 ml/min run (3,470 cm/hr) in reversed- phase mode. |