FYI Note

FYI042298-05

STYROS™ Polymeric Stationary Phases as Compared with Silica.

Rather than spending hours finding the optimum conditions to separate your proteins, using reversed phase silica:

You could spend minutes using  STYROS™  reversed phase polymerics:

And then reduce the flow rate to find out more about additional details, such as the effect of reversed phase media, time or organic solvent, on your proteins:

Operating parameters: 

	HPLC System	HP 1100, Standard Cell
	Detector	214 nm
	Column	As indicated on each chromatogram
	Mobile Phase	A: 0.1 % TFA in H2O B: 0.1 % TFA in Acetoniltrile/H2O (95/5)(v/v)
	Gradient	15 to 100 % B in 30 Column Volume
	Flow rate	Chromatogram 1:  2 ml/min(3,470 cm/h) Chromatogram 2:  3ml/min (1,080 cm/hr)
	Temperature	20°C
	Injection volume	6ml for 2.1 x 150 mm column 10 ml for 4.6 x 50 mm column
	Sample:	Ribonuclease, Cytochrome C, Lysozyme, BSA, Myoglobin, OVA.

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