SIMULATED MONOLITH™ POLYMERIC STATIONARY PHASES FOR LIQUID CHROMATOGRAPHY
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Application Notes
1
Comparison of STYROS™ 1R and 2R with a Silica C4 Stationary Phase.
2
Fast Protein Separation on STYROS™ 1R and 2R.
3
Comparison of STYROS™ 1R/XP and 1R/XH.
4
Separation of Angiotensin Variants at Basic pH.
5
High Performance Separation of Proteins on STYROS™ 1R/NB
6
Ultra Fast Separation of Proteins on Narrow Bore Columns.
7
Quality Control of Proteins of Different Lots from the Same Supplier.
8
Assessing Proteins Across Grades and Suppliers.
9
Polymeric Hard Gel Media-HPLC vs. Soft Gel-FPLC in the Separation of Biomolecules.
10
Fast Separation of Protein Isoforms with Fully Porous Hard Gel Media: Hemoglobins.
11
Polymeric Gigaporous Strong Anion Exchangers: Bead Size Distribution versus Pore Size Distribution.
12
Polymeric Gigaporous Strong Cation Exchangers: Bead Size Distribution versus Pore Size Distribution.
13
High Speed Resolution: Efficient Use of Long, Narrow-bore Columns.
14
Polymeric Gigaporous Strong Cation Exchangers: Effect of Capacity on Protein Resolution.
15
Separation of IgG from Albumins in Commercial Production.
16
Polymeric Gigaporous Anion Exchanger: Throughpores versus Superficial Pores.
17
Polymeric Gigaporous Anion Exchanger :
Performance
at Low Flow Rates.
18
Separation of Small Molecules on STYROS
™
Q/XH.
19
Resolving Efficiency of Longer Columns: case application with medium capacity cation exchanger.
20
Metal Chelate Liquid Chromatography on Hard Gel Gigaporous Polymeric Media: Comparison with Soft Gel.
21
Metal Chelate Liquid Chromatography on Hard Gel Gigaporous Polymeric Media: Tri-dentate IDA versus Five-dentate TED
22
Separation of Cytochrome c Isoform on
STYROS
™
SE
: Strong cation exchanger.
23
Fast Separation of IgG on STYROS
™
HQ: High Capacity Polymeric Gigaporous Strong Anion Exchanger
.
24
Fast Separation of IgG, IgA and IgM on STYROS
™
DEAE/NB: Narrow Bore Weak Anion Exchanger.
25
Fast Detection of XELODA (Capecitabine) in Urine Sample with STYROS
™
2R/XH.
26
StyrosZyme
™
TPCK-Trypsin, Immobilized Enzyme on Polymeric Hard Gel Stationary Phase: Online Digestion of Lysozyme in 5 minutes
.
27
StyrosZyme
™
TPCK-Trypsin, Immobilized Enzyme on Polymeric Hard Gel Stationary Phase: Online Digestion of Insulin Oxidized B-chain in 16 minutes.
28
StyrosZyme
™
Pepsin, Immobilized Enzyme on Polymeric Hard Gel Stationary Phase: On line digestion of Cytochrome c From Horse Heart and Bovine Heart.
29
SIMULATED MONOLITH™: Fast Separations with HIC (Hydrophobic Interaction Chromatography)
30
SIMULATED MONOLITH
™
:
Comparison With Non Covalently Coated Polymeric Hard Gel.
31
StyrosZyme
™
Papain, Immobilized Enzyme on Simulated Monolith Polymeric Stationary Phase:
Effect of Temperature and Linear Velocity on Online Digestion.
32
Rapid isolation of IgG From Human Serum on STYROS
™
rA
(Immobilized Recombinant Protein A on Simulated Monolith Polymeric Stationary Phase).
33
ADRIAMYCIN: Detection in Urine Sample with STYROS™ 2R/XH
34
Immobilized Protein rA on Polymeric Porous Hard Gel (STYROS
™
rA): Comparison with Sepharose rA.
35
NAVELBINE: Fast Detection in Urine Sample with STYROS
™
2R/XH.
36
On Line Affinity capture of APROTININ on StyrosZyme™ TPCK-Trypsin, Immobilized Enzyme on Polymeric Hard Gel Stationary Phase.
37
Separation of Aprotinin from apo-Transferrin and Hexokinase on Polymeric Hard Gel Anion-Exchange Columns.
38
Aprotinin Purity Test on STYROS™ Anion and Cation Exchanger. Polymeric Hard Gel Stationary Phase.
39
StyrosZyme™ Pepsin. Immobilized Enzyme on Polymeric Hard Gel Stationary Phase: On line digestion at 0º C and pH 2.5.
40
Hydrophobic Interaction Chromatography compared with Polymeric Reversed Phase: STYROS
™
HIC-Butyl versus STYROS
™
2R.
41
Hydrophobic Interaction Chromatography : Comparison of STYROS
™
HIC-Phenyl with Shodex HIC PH-818.
42
Reversed Phase Polymeric: Comparison
of STYROS
™
2R with Commercial Non Porous Polymeric.
43
Hydrophobic Interaction Chromatography: Comparison of STYROS
™
HIC-Phenyl with TSKgel Phenyl-5PW from TOSOH
.
44
Hydrophobic Interaction Chromatography: Comparison of STYROS
™
HIC-Butyl with TSKgel Butyl-NPR from TOSOH.
45
Hydrophobic Interaction Chromatography: Comparison of STYROS
™
HIC-Ether with TSKgel Ether-5PW from TOSOH.
46
Separation of Hen Egg White Proteins: STYROS™ HQ Compared with Mono Q
48
Low Salt Separations on Shielded Weak Anion Exchanger: STYROS
™
SWAX
49
STYROS
™
3R Simulated Monolith
™
Polymeric Reversed Phase.
50
STYROS™ 3R Simulated Monolith
™
Polymeric Reversed Phase: Standard Separation of 6 Small Molecules
51
STYROS
™
Amino HILIC Simulated Monolith
™
Polymeric Normal Phase: Standard Separations.
52
STYROS
™
Amino HILIC Simulated Monolith™ Polymeric: Separation of Benzoic Acid and Derivatives
53
STYROS
™
Amino HILIC Simulated Monolith™ Polymeric:
Effect of Ionic Strength and Temperature on the Separation of Aromatic Acids
54
STYROS
™
Amino HILIC Simulated Monolith
™
Polymeric: Separation of Angiotensins I, II and III
55
STYROS
™
HILIC Simulated Monolith
™
Polymeric Normal Phase: S
tandard Separations
56
STYROS
™
HILIC Simulated Monolith
™
Polymeric Normal Phase:
Separation of Aliphatic and Aromatic Acids
57
STYROS
™
HILIC Simulated Monolith
™
Polymeric Normal Phase:
Separation of Nucleotides
FYI Notes
1
Reversed Phase and Ion Exchange Chromatography of Two proteins on STYROS
™
Columns.
2
Effect of Long Residency Time and Heat During Reversed Phase Chromatography of Some Proteins
.
3
A Simple Alternative to Reduce Sample Consumption and Waste Generation by Over 40 %.
4
Resolving Capabilities of a 25 cm Column Without the Flow and pH Restriction of Silica.
5
STYROS™ Polymeric Stationary Phases as Compared with Silica.